Colorectal cancer is one of the most common disease worldwide and has a great mortality potential. Chronic inflammation is induced by biological, chemical and physical factors and it’s related with increased cancer risk in human. The studies have revealed that some proteins of Annexin family may play anti-inflammatory roles. Between these proteins, decreased Annexin V levels are known to inhibit the effects of inflammatory mediators such as secretory phospholipase A2 and Interleukine-1.
In the present study, we aimed to evaluate the relation between Annexin V protein and some inflammatory mediators in colorectal cancer.
Forty-five colorectal cancer patients were included in the study and 46 healthy volunteers formed our control group. The quantitative determination of plasma interleukin-6, interlekin-1B and arachidonic acid were performed by enzyme-linked immunosorbent assay. Annexin V mRNA expression analysis was performed by Real Time PCR.
We found significantly increased IL-6 (1.87±0.75 pg/ml), and arachidonic acid (735.55±23.37 ng/ml) levels in patient group when compared to control group (0.56±0.35 pg/ml, 706.27±55.56 ng/ml, respectively, p<0.05). We also observed a significant decrease of Annexin V mRNA expression in patient group with 27.42±2.62 Ct values (p<0.05). We found significant negative correlation between AnxV expression and arachidonic acid levels (p=0.029). The IL-1B levels of patients were not different when compared to control.
The results indicate the inflammatory state in colorectal cancer patients. The reduced AnxV mRNA expression and the significant correlation between AnxV and inflammatory mediator strength the hypothesis that Annexin V protein may be an important marker on development of inflammation in colorectal cancer. The further studies will be more elucidative on evaluating the hypothesis.
The study was supported by Ankara University Research Foundation (2013-13B3336002).