Background:Cervical cancer is a malignant neoplasm arising from cells originating in the cervix uteri.Human papillomavirus (HPV) infection appears to be a necessary factor in the development of almost all cases (more than 90%) of cervical cancer.
Aim: The aim of this study was to select the more suitable testing method for early screening of uterine cervical cancer to protect susceptible populations．
Methods: We used two methods to detect the infection status of 13 high risk HPV types during women’s health screening．The study group consisting of 879 healthy women was divided into two groups according．One was the low age group(<40 years old)，and the other was the high age group(≥40 years old)．
Results:By fluorescence quantization PCR, the total high risk HPV-positive rate for the entire study group was 15.93% (140/879)．By fluorescence quantization PCR, the positive rate was 10.2% (19/186)for the low age group，while the positive rate was 17.5% (121/693) for the high age group (P<0.05)．The total detected high risk HPV-positive rate by HC2-HPV-DNA was 11.83% (104/879). By HC2-HPV-DNA, the positive rate was 10.2% (19/186) for the low age group，while the positive rate was 12.3%(85/693) for the high age group. The positive and negative rates for combination of fluorescence quantization PCR and HC2-HPV-DNA were 9.56% (84/879) and 81.80% (719/879), respectively. Statistical difference was observed between fluorescence quantization PCR and HC2-HPV-DNA in detecting high risk HPV types (P<0.05).
Conclusions:Women over 40 years old have a high risk for high risk HPV infection．High risk HPV detection methods must be enhanced to decrease the incidence and mortality of cervical cancer in women over 40 years old. The HC2-HPV-DNA is superior to fluorescence quantitation PCR for detecting 13 high risk HPV types and is more suitable for large scale health screening.