Human papillomavirus (HPV)infection, is a major risk factor for the development
of cervical cancer worldwide, particularly HPV-16.A strong association has been
shown between viral load of human HPV-16 and the progress of cervical lesions.
However, little data is available for the significance of viral load in
prognostic of cervical cancer.
In this study,we demonstrated relationship between viral load of HPV 16infection and cervical cancer,and its potential clinical significance as a prognostic factor.
Two hundred-ten human papillomavirus 16-positive patients who underwent radical
hysterectomy with pelvic lymphadenectomy for FIGO stage IA2-IIA cervical cancer
were recruited in this study.Viral load measurements of HPV16 were identified
by qRT-PCR.The association of viral load with clinicopathologic characteristics
and overall survivals were analyzed.
Viral load measurements of HPV-16 were detected in all of 210 evaluable cases,
and the results of correlation analysis indicated that HPV16 viral load was
significant related with cervical cancer patients age (P= 0.013), FIGO stage
(P =0.024),and lymph node metastasis (P=0.041).Univariate analysis showed that
the patient's age, viral load of HPV-16, FIGO stage, histological grade, lymph
node metastasis were the poor factors for overall survival time (P <0.05).
Kaplan-meier survival analysis showed that the overall 5-year survival rates of
the viral copy number range 107-109,105-106,103-104 of HPV16 were 34.4%, 48.0%
and 87.1%, respectively (P <0.05). Cox regression analysis showed that lymph
node metastasis (OR=9.617; 95% CI 1.598-7.719; p=0.002) , viral load of HPV-16
(OR=12.403; 95% CI 1.628-5.535; p=0.001) are independent prognostic factors.
Our findings suggest that viral load of HPV-16 is an independent reliable
prognostic factor of early-stage cervical cancer.