Colorectal cancer (CRC) is one of the leading causes of cancer-related deaths worldwide. CRC have been linked to several genetic variants such as, K-RAS, p53, PTEN, and MLH1 and MSH2 genes
The aim of the present study was to assess, the alteration of K-RAS, p53, PTEN, and MLH1, in colorectal cancer tissues obtained from Saudi patients.
Fifty Formalin fixed paraffin wax processed colorectal samples were retrieved from histopathology Laboratory. Five micron sections were obtained and immunostained, using antibodies to K-RAS, p53, PTEN, and MLH1.
Of the 50 cases, 76% were adenocarcinomas, 14% invasive adenocarcinomas and 10% Mucinous carcinomas. Of the carcinomas 93% were identified in Stage II and 7% in Stage III Positive immunohistochemical expressions of K-RAS and p53 were identified in 38% and 54%, respectively, Lack of expressions were found in 21% and 11% in this order.
There is a considerable genetic variation in for K-RAS, p53, PTEN, and MLH1 genes among Saudi patients with colorectal cancer. Immunohistochemical testing for K-RAS, p53, PTEN, and MLH1 expression which provides prognostic information for management of patients with colorectal cancer.